What is Knockout Animals ? - Part 2- Science 8

A cat sitting on the chair, just for illustration

Previous posting (Part 1):

- The Rainbow Before Evening : What is Knockout Animals ? - Part 1- Science 8

Sure, a knocked out gene has two sides:

- advantages
- disadvantages

One of important advantages is that a researcher knows which factor precisely in determining animal behavior, in this case a certain behavior of mice.

Another advantage, researchers could decide the exact time to disrupt a specific gene. The timing is important to conduct behavior experiments in the laboratory.

However, there are several disadvantages, some of them:

1) Expensive economically

- a lot of labors (labor consuming) include culturing of stem cell

2) Gene knockout may lethal to embryo development.

- hence, animal can’t reach adulthood.

3) May incomplete knockout process

4) Disruption of genes may overlap.

5) A knockout gene may develop cancer in certain tissues of mice.

# Previous postings:

- Rediscovering of Rare Blue Bee in Florida in 2020 – Science 3
- Can Scientists Create Glowing Plants? - Science 4
- The Amphibians Near Extinct due to Fungus Disease – Science 5
- Can Succulents Improve Room Humidity? - Science 6

Outlawed - An Epic Western Novel – Part 2 - Reading 4

Red rose in the garden

Previous posting (Part 1):

- Outlawed - An Epic Western Novel – Part 1 - Reading 4

Her mother begins to worry about Ada.

She finds a man to “help” Ada to get pregnant and said not to think about unfaithful to her husband, but to keep herself safe.

Almost one year is over, and Ada is not pregnant yet.

Then she has been kicked out from her husband and husband’s family where she lives with.

Ada comes back to her mother and younger sisters.

German measles outbreaks and the disease attacks pregnant women and babies.

The folks believe that the deaths and miscarriages caused by witchcrafts, barren women, and one of them is Ada.

Ada must leave the town, then she lives in covenant.

Anna North, the author of Outlawed.

When she was in covenant she learns about the causes of infertility, then learned how to solve it..

She has great knowledge about herbal, frontier medicine and midwifery.

Then she joins a band of outlaws led by a preacher-turned-robber known to all as the Kid, the Hole in the Wall Gang.

Ada learns how to:

- rob highway
- steal cattle
- appear and behave like a binary man.

However, she finds that the gang is the safe place for barren women and gender nonconforming people.

# Previous postings:

- The Rainbow Before Evening : Like Crazy, “a wild child cares for the wild woman who raised him” - Reading 2

What is Knockout Animals ? - Part 1- Science 8

Knockout mice, credit to National Human Genome Research Institute

We might confuse with these two terms:

- knockout
- Transgenic.

Transgenic is insertion of exogenous DNA into random non homologous site in the animal genome.

While Knockout is insertion of modified DNA at a specific homologous site in the animal genome.

It is interesting to discuss about knockout term furthermore in this posting.

In addition, knockout is the word commonly used to inactive an exist of specific gene in particular organism.

The way to knocked out is by replacing or disrupting the target gene with an artificial piece of DNA.

This is a genetic technique by using homologous recombination to change an endogenous gene.

Garden corner, just illustration

Then, targeted gene could be permanent or conditional inactive or knockout.

Knockout could cause alter phenotype of organisms.

The changes might include behavior, appearance (observable physical) and biochemical characteristics of organisms.

Then, knockout organisms are used in learning about a gene that has been sequenced, which incompletely known function.

# To be continued to Part 2

# Previous postings:

- The Rainbow Before Evening : Making Perfume in the Organic Chemistry Laboratory - Science 1
- The Rainbow Before Evening : Russian Covid 19 Vaccine Advantages – Science 2

Outlawed - An Epic Western Novel – Part 1 - Reading 4

Cover of Outlawed, a Western fiction

Outlawed is a Western fiction by Anna North.

The writer grown up in Los Angeles and lives in Brooklyn. She was a graduate of Iowa Writer’s Workshop.

Ada is the protagonist in this book. The story is set in the late of nineteenth century.

From the front jacket of this book, “In the year of our Lord 1894, I became an outlaw. Like a lot of things, it didn’t happen all at once.”

The story begins when Ada feels lucky to get married with a handsome boy from the good family.

Ada’s mother is a midwife and Ada always happy to help her mother doing her job.

Like other her peers, she married at seventeen, and she loves her husband.

Their duty is to have children for baby Jesus since God sent the Great Flu (flu pandemic) to cleanse the world of evil.

Several months after they wed, Ada’s womb is still empty. Meanwhile in her town, the barren women are routinely hanged as witches.

# To be continued to Part 2

## Previous posting:

- Family Saga and Meaning to be a Good Person – Part 1 - Reading 1
- Family Saga and Meaning to be a Good Person – Part 2 - Reading 1

When should I get the Botox Cosmetic? - Part 2 – Healthy Life 16

The smile of camels (Credit: Fayez Nureldine/AFP/Getty Images).

Previous posting:

- When should I get the Botox Cosmetic? - Part 1 – Healthy Life 16

Do you think someone has bad reputation or cheat on you whenever she get Botox injection in her forehead or her face?

Finally I found it out.

It was said, “If Botox purpose to get $55 million dollar contest the most beauty camel in King Abdulaziz Camel Festival, forget it!!!”

The Botox injection into camels like a cheat in this contest.

I'm not going to cheat anyone, even with my face.

I'm thinking about a prevention, before the frown lines or “eleven” lines between my eyebrows are exist in my forehead, so that I will not look:

- angry
- tired
- or unhappy.

Trees nearby jogging track.

One of medical practitioners said that if you think that you have inherit the early frown lines or wrinkles, do not wait the wrinkle create the lines.

So in this case, Botox treatment is the best under 30 year old, because Botox is not a filler. Botox works to paralyze muscles and need to be repeated.

What do you think, do you think in age before 30 year-old is too early to get Botox Cosmetic?

Or just wait until the lines between the eyebrows and forehead are appearing?

# Previous postings:

- Measles Outbreak in a Recent year – Part 1 – Healthy Life 11
- Measles Outbreak in a Recent year – Part 2 – Healthy Life 11
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- Do you know about E. coli Bacteria? - part 2 - Healthy Life 12

The Surprising Fact - Cooked Shrimps are Cheaper than Raw Ones – Part 2 - Food 8

Plastic bags of seafood market and deli

Previous post (part 1):

- The Surprising Fact - Cooked Shrimps are Cheaper than Raw Ones – Part 1 - Food 8

When I was in the market, I saw both the raw and cooked shrimps.

The shrimps either plain cooked or cooked with recipes, Louisiana special cuisine.

Off course!

What surprising me was the price, you may compare them:

- cooked shrimps are US$ 9.99 per pound
- whereas raw ones are US$ 11.99 per pound.

Cooked shrimps are two dollar cheaper than raw ones.

Why?

I really don’t know the exact or true answer, what happen behind the prices, honestly.

However, I guess there are several factors caused cooked shrimp cheaper than raw or fresh ones.

Cooked shrimps

Here they are the answers:

1) about to expire or go bad

- the sellers want to get rid of it as soon as possible.

2) smaller size

- bigger size is expensive

I decided to buy cooked shrimps together with boiled corn. Ready to go and to eat.

Make me wonder, did you find in your place where cooked things are cheaper than raw ones?

# Previous posting:

- The Rainbow Before Evening : Green Bean and Papaya Delight – Food 4
- The Rainbow Before Evening : The Best Steakhouse in my City, Louisiana – Food 5
- The Rainbow Before Evening : Have You Tasted Matcha Latte with Soy Milk or Almond Milk? - Food 6

When should I get the Botox Cosmetic? - Part 1 – Healthy Life 16

Botox Cosmetic Injections (Credit: Noravector/123rf.com)

Every time I get out from my house, I always pass the nice building with big signs of the professional names in the medical fields, the services, and the treatments.

The building is located at the intersect of a quite drive and a busy traffics with 5 traffic lines.

The nice building looks not so busy among the:

- restaurants
- bakery
- and sandwich fast food.

Because the parking lot is in rear of the building, the clients get in and out with the good manners through the quite drive.

There is a Cosmetic Center that serves vary of treatments, including cosmetic surgeries, hair loss replacement, and medical spa.

Then?

Cup cakes, just illustration.

The services are all what we need to treat and fix in our hair, eyes, face, body, breast, and skin.

The building is okay, nothing wrong. The led signs are still blinking with the facilities and services. I knew that this Cosmetic Center is the first Botox Cosmetic in my state.

Lately, I just have an imagination about one of their services, Botox, and the smile of camels.

Sounds good to me, Botox Cosmetic for the human beauty. I just wondered about the beauty of camel last year. How come camels got Botox Cosmetic and had a negative reputations.

# To be continued to Part 2