Thursday, 14 October 2021

What is Knockout Animals ? - Part 1- Science 8

Knockout mice, credit to National Human Genome Research Institute

We might confuse with these two terms:

- knockout
- Transgenic.

Transgenic is insertion of exogenous DNA into random non homologous site in the animal genome.

While Knockout is insertion of modified DNA at a specific homologous site in the animal genome.

It is interesting to discuss about knockout term furthermore in this posting.

In addition, knockout is the word commonly used to inactive an exist of specific gene in particular organism.

The way to knocked out is by replacing or disrupting the target gene with an artificial piece of DNA.

This is a genetic technique by using homologous recombination to change an endogenous gene.


Garden corner, just illustration

Then, targeted gene could be permanent or conditional inactive or knockout.

Knockout could cause alter phenotype of organisms.

The changes might include behavior, appearance (observable physical) and biochemical characteristics of organisms.

Then, knockout organisms are used in learning about a gene that has been sequenced, which incompletely known function.

# To be continued to Part 2

# Previous postings:

21 comments:

  1. Animals always serve as guinea pigs for science we owe them a lot!

    Good night wishes!
    A hug, friend

    ReplyDelete
    Replies
    1. Thanks-
      What is Knockout Animals ? - Part 1- Science 8
      -

      Delete
  2. Hi, dear, thanks for visiting, I'm here to pay you back, I'm in a hurry today, but I'll be back more often.
    A hug and good night

    ReplyDelete
    Replies
    1. Thanks-
      What is Knockout Animals ? - Part 1- Science 8
      -
      Knockout mice, credit to National Human Genome Research Institute

      We might confuse with these two terms:
      - knockout
      - Transgenic.

      Transgenic is insertion of exogenous DNA into random non homologous site in the animal genome.

      While Knockout is insertion of modified DNA at a specific homologous site in the animal genome.

      It is interesting to discuss about knockout term furthermore in this posting.

      In addition, knockout is the word commonly used to inactive an exist of specific gene in particular organism.

      The way to knocked out is by replacing or disrupting the target gene with an artificial piece of DNA.

      This is a genetic technique by using homologous recombination to change an endogenous gene.

      Delete
  3. Replies
    1. Thanks-
      What is Knockout Animals ? - Part 1- Science 8
      -
      Garden corner, just illustration

      Then, targeted gene could be permanent or conditional inactive or knockout.

      Knockout could cause alter phenotype of organisms.

      The changes might include behavior, appearance (observable physical) and biochemical characteristics of organisms.

      Then, knockout organisms are used in learning about a gene that has been sequenced, which incompletely known function.

      # To be continued to Part 2

      # Previous postings:

      - The Rainbow Before Evening : Making Perfume in the Organic Chemistry Laboratory - Science 1
      - The Rainbow Before Evening : Russian Covid 19 Vaccine Advantages – Science 2

      Delete
  4. Replies
    1. Thanks-
      What is Knockout Animals ? - Part 1- Science 8

      Delete
  5. Realmente muito interessante!
    Te desejo um feliz fim de semana minha amiga!
    Abraço

    ReplyDelete
    Replies
    1. Thanks-
      What is Knockout Animals ? - Part 1- Science 8
      -
      Knockout mice, credit to National Human Genome Research Institute

      We might confuse with these two terms:
      - knockout
      - Transgenic.

      Transgenic is insertion of exogenous DNA into random non homologous site in the animal genome.

      While Knockout is insertion of modified DNA at a specific homologous site in the animal genome.

      It is interesting to discuss about knockout term furthermore in this posting.

      In addition, knockout is the word commonly used to inactive an exist of specific gene in particular organism.

      The way to knocked out is by replacing or disrupting the target gene with an artificial piece of DNA.

      This is a genetic technique by using homologous recombination to change an endogenous gene.

      Delete
  6. Olá, amiga!
    Interessante e oportuna informação.
    Votos de um excelente fim de semana!
    Beijinhos!

    Mário Margaride

    http://poesiaaquiesta.blogspot.com

    ReplyDelete
    Replies
    1. Thanks-
      What is Knockout Animals ? - Part 1- Science 8
      -
      Garden corner, just illustration

      Then, targeted gene could be permanent or conditional inactive or knockout.

      Knockout could cause alter phenotype of organisms.

      The changes might include behavior, appearance (observable physical) and biochemical characteristics of organisms.

      Then, knockout organisms are used in learning about a gene that has been sequenced, which incompletely known function.

      # To be continued to Part 2

      # Previous postings:

      - The Rainbow Before Evening : Making Perfume in the Organic Chemistry Laboratory - Science 1
      - The Rainbow Before Evening : Russian Covid 19 Vaccine Advantages – Science 2

      Delete
  7. Grata pela interessante informação!

    ReplyDelete
  8. A ciência está sempre em constante evolução.
    A temática é fascinante, mas é uma área acerca da qual tenho conhecimentos reduzidos.
    Abraço amigo.
    Juvenal Nunes

    ReplyDelete
    Replies
    1. Thanks-
      What is Knockout Animals ? - Part 1- Science 8
      -
      Garden corner, just illustration

      Then, targeted gene could be permanent or conditional inactive or knockout.

      Knockout could cause alter phenotype of organisms.

      The changes might include behavior, appearance (observable physical) and biochemical characteristics of organisms.

      Then, knockout organisms are used in learning about a gene that has been sequenced, which incompletely known function.

      # To be continued to Part 2

      # Previous postings:

      - The Rainbow Before Evening : Making Perfume in the Organic Chemistry Laboratory - Science 1
      - The Rainbow Before Evening : Russian Covid 19 Vaccine Advantages – Science 2

      Delete
  9. Muy interesante la información.

    Un lindo fin de semana.
    Abrazo

    ReplyDelete
    Replies
    1. What is Knockout Animals ? - Part 1- Science 8

      Delete
  10. Thanks-
    What is Knockout Animals ? - Part 1- Science 8
    -
    Knockout mice, credit to National Human Genome Research Institute

    We might confuse with these two terms:
    - knockout
    - Transgenic.

    Transgenic is insertion of exogenous DNA into random non homologous site in the animal genome.

    While Knockout is insertion of modified DNA at a specific homologous site in the animal genome.

    It is interesting to discuss about knockout term furthermore in this posting.

    In addition, knockout is the word commonly used to inactive an exist of specific gene in particular organism.

    The way to knocked out is by replacing or disrupting the target gene with an artificial piece of DNA.

    This is a genetic technique by using homologous recombination to change an endogenous gene.

    ReplyDelete
  11. Importante el tema de la ciencia. un abrazo

    ReplyDelete
    Replies
    1. Thanks-
      What is Knockout Animals ? - Part 1- Science 8
      -
      Knockout mice, credit to National Human Genome Research Institute

      We might confuse with these two terms:
      - knockout
      - Transgenic.

      Transgenic is insertion of exogenous DNA into random non homologous site in the animal genome.

      While Knockout is insertion of modified DNA at a specific homologous site in the animal genome.

      It is interesting to discuss about knockout term furthermore in this posting.

      In addition, knockout is the word commonly used to inactive an exist of specific gene in particular organism.

      The way to knocked out is by replacing or disrupting the target gene with an artificial piece of DNA.

      This is a genetic technique by using homologous recombination to change an endogenous gene.

      Delete
  12. new for me What is Knockout Animals ? - Part 1- Science 8

    ReplyDelete

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